Peak Resolution Calculation Formula


In chromatography, the resolution is a measure of the separation of two peaks of different retention time in a chromatogram. It is measured by dividing the difference in peak retention times by the average peak width.



Usp resolution calculation formula

The resolution between peaks of two components may be calculated using the following equation:

RS (resolution) = 1.18 (tR2− tR1) ÷ (Wh1+Wh2)

Where,

tR2>tR1

tR1, tR2 = retention times of the peaks

Wh1, Wh2= peak widths at half-height


In quantitative thin-layer chromatography, using densitometry, the migration distances are used instead of retention times and the resolution between peaks of two components may be calculated using the following equation:

RS = 1.18a (RF2−RF1) ÷ (Wh1+Wh2)

Where,

RF2>RF1

RF1, RF2 = retardation factors of the peaks

Wh1, Wh2 = peak widths at half-height

a = migration distance of the solvent front


Read also:

Post a Comment

أحدث أقدم